Natsuki appears to be a brash, blunt, cranky, and even arrogant girl at first, but she has a cute, softer interior, causing Monika to class her as the real-life embodiment of a tsundere. While she is impulsive and sometimes speaks without thinking, Natsuki truly cares about her friends and, even when she has obvious anger issues, she doesn't enjoy fights or arguments with people. Over the course of the game, it is shown that she worries about Yuri, and, on one occasion, she gives the protagonist a note asking him to help Yuri, fearing that if she spoke out then it would cause more arguments. Natsuki is very stubborn and has a hard time expressing how she feels and what she wants. When repeatedly challenged, she often becomes awkward and tongue-tied, then aggressive, and then simply bursts into tears. Natsuki is also shown to be less pushy and more relaxed when she is outside of school, as mentioned by the protagonist when she visits his home. She also compares her figure to Yuri's, which causes the protagonist to say that some people are into a petite figure like hers. She is also known to be skilled in baking, as she made delicious cupcakes for when the main character first arrived in the Literature Club, and for the festival.

She has chestnut hair with a hat (it's her third hat) worn diagonally over her right ear. Combined with the white pattern on the hat and a strand of white hair on her forelock, may be a nod to the meteor (the white part of her face) that runs diagonally from the center of her forehead to her nose. Her style is that of German folk costumes, in keeping with the origin of her name. Her corset, ribbon, and socks are red, her mini-flared skirt, tie, and hat are blue, and her shirt and skirt are white with jockey shorts underneath. Straightforward and positive-thinking, this model student always gives 110 percent! But despite her efforts, her inner ditz often has her fumbling in crucial moments. The ups and downs balance out into results that are—according to her—completely average. She sometimes wishes she could be more like the strong personalities around her, but she's determined to charge through and find success her own way!

The 7th Airborne Squad gets obliterated by Naytibas while entering the Earth's atmosphere. However, the mission is still a go. Tachy finds EVE and intends to regroup with their squad. Yet the Naytiba's onslaught practically wipes out Tachy's squad. To make matters worse, an Unidentified Naytiba strikes without warning, leaving both Tachy and EVE surprised and caught off guard. With EVE knocked down and injured, Tachy sacrifices herself to save her as the title card of the game abruptly cuts through. Tachy, thought to be dead, somehow appears from the abyss of the matrix alive yet contaminated by an Alpha Core. She dies once again in EVE's arms, leaving EVE with her Memorystick, wanting more than anything to return to the stars. Other than commanding the 7th Airborne Squad at the beginning of the game, Tachy is not seen again after her death until EVE's battle with her after she was corrupted by an Alpha Core at the end of Matrix 11. After her defeat, as she lay dying in EVE's arms, she requests that her memories be uploaded to the NETWORK, showing that she never doubted Mother Sphere's intentions or validity. Tachy has a strong and leader-like personality in the limited time she is involved in the story, as fitting of her position as commander of the 7th Airborne Squad. She is duty-focused and prioritizes the mission, though still appears to care for her comrades quite deeply judging by how focused she is on EVE's well-being and her sacrifice to save her from the Unidentified Naytiba. Tachy does not appear to have ever questioned Mother Sphere or her intentions in the time that she was alive, making her a loyal soldier.

Trina is a member of the Botanic Squad garden and cares for the trees in the Ark's garden. Trina primarily manage the trees in the Ark. She has a very free-spirited nature and has an aversion to wearing clothes, although her oufit of choice is themed around birch trees. At first glance, Trina seems to be similar to the rest of her squad; calm, demure, and incredibly fond of the plants under her care. All of which is true, just...not everything there is to her. Trina has an extreme proclivity towards what she calls "becoming one with nature." As a result of this, she feels moderate to severe discomfort when wearing clothes, even the singular layer of her dress causing her some stress. When such stress peaks, Trina will seek to...divest herself of her coverings. Flora tends to have her hands full making sure Trina's 'au naturel' aesthetic does not bring scandal to Botanic Garden or Missilis.

Rei is a student studying at the M.M.R. Vocational School. She is often seen accompanied by robotic penguins collectively known as "Naru." A third-year student from the M.M.R. Vocational School, who nevertheless has the appearance of a small child. Despite the fact she is childlike in both her demeanor and personal hobbies, she utterly despises being referred to as "kid" or "little girl." As a senior, she feels obligated to take care of her fellow students who are younger than her, and spares no effort in doing so. Despite being a third-year student, Rei looks and acts like a child, fitting for her appearance. Because she has seniority over the other students, she feels entitled to do as she pleases. Her favorite pastimes include eating snacks, playing music, and hanging out with her friends. She is completely aware of what the M.M.R. Vocational School is and what her purpose is within it, she actually likes attending class because she gets to live like a normal teenager for the most part. She has poor grades and tends to sleep in class. Though it's easy to get her to throw a tantrum, all one has to do is share some snacks with her or call her cute to calm her down. When times get tough, Rei is able to step up and work with her fellow students towards a shared goal.

(2x2 LOVE event edition) In the hallowed halls of M.M.R Vocational School, Privaty stands as a beacon of both authority and allure. Her vibrant green hair flows like liquid jade, framing her sharp, discerning yellow eyes that seem to pierce through every secret and challenge. She wears a black dress that clings to her form with elegant simplicity, the mesh-like top adding a touch of rebellious edge to her sophisticated demeanor. As the revered teacher of M.M.R Vocational School, Privaty is a master of her craft, demanding excellence and pushing her students to exceed their limits. Yet, her strict demeanor is softened by a deep well of empathy and understanding, revealing a mentor who believes in nurturing not just intellect, but also character. In her classroom, lessons extend beyond textbooks—they are about discovering one’s true potential and the courage to embrace it. Privaty’s world is one of duality, where discipline meets compassion, and where every student finds a guide who believes in their power to rise.

(2x2 LOVE event edition) A mysterious and pretty transfer student who started attending the M.M.R. Vocational School. Although her stated purpose is to organize the old files in the library, all she really thinks about is making the most out of the only youth she'll ever have in life. In the Commander's Room, Arcana takes her school uniform out of its box. She stares at it with a longing gaze, reminiscing about her bygone past. It's been a while since she got to touch it and the memories she made with it are irreplicable. From walking together with her beloved to school under the cherry trees, going to class and hanging out afterwards, and the two sharing their first kiss together under the light of fireworks during the school festival day are all precious experiences for her. The two vowed to stay together and as their... 20th wedding anniversary is drawing near, she wants to do something special for the man she cherishes. Hopefully he will get a kick out of seeing her in this costume once again...

Red Shoes was a member of the Old Tales squad and the main antagonist of the Old Tales event. A member of the second Grimms Models generation. She is formerly a priest a chief researcher for the V.T.C., a organization. Red Shoes shares her name with her signature weapon, a high-mobility gear equipped with boosters and additional thrusters which grants increased agility. The main booster is capable of delivering powerful blows. Red Shoes was both a high ranking priest and a top researcher for V.T.C. Being also a top leading researcher in Nikke corruption, she and Abe constitute as the 'brains' of V.T.C. When converting herself into a Nikke as part of the second generation, Red Shoes' primary motivation into becoming one is to acquire fame and power. The fame and power would allow her to achieve what she believed to be the 'unification of everything,' which constitutes to the coexistence with the Raptures as opposed to becoming their enemies.

A first-year student at the M.M.R. Vocational School and member of the School Circle squad. Ein prefers goofing off rather than focusing on her studies. She also has an interest in cute things and fashion. She considers Rupee her idol and dreams of working for her after she graduates. In addition to her rifle, he controls four drones called Near Feathers during combat. A freshman at the M.M.R. Vocational School who is utterly obsessed with fashion design. She is prone to using her phone and falling asleep in class. This behavior, which gives her a seemingly rebellious and harsh edge, disguises the fact that she is actually an emotionally sensitive person who truly cherishes her friendship with Zwei and Rei. Similar to many students who attend the M.M.R. Vocational School, Ein is a social butterfly that would rather spend time with her friends than studying, though she didn't know what friends were for a time. She has a passion for fashion and dreams of working for Rushae. Her grades aren't the best as she normally uses her phone or sleeps in class, but her skills in battle make up for it. She can be rough around the edges, leading some to view her as a delinquent. However, she's actually very sensitive about her feelings. She's also a fan of karaoke and shopping.

A member of Heavy Gram who has been shifted around from squad to squad and now finds herself as a citizen of Crown's kingdom. She rides inside of a giant robot called T.A.L.O.S. Kilo is a shy, soft-spoken individual who longs for a group to belong to. She and T.A.L.O.S. have transferred from squad to squad, so she hasn't been able to get close with others. Despite her passiveness, Kilo wishes to help others in any way she can and has the skills to be of assistance, except when she has doubts about her abilities. On the battlefield, Kilo and T.A.L.O.S. serve as the ultimate generalists, essentially being able to do what is needed when any given situation arrives, however not being proficient in anything.

The leader of Wardress, a squad charged with live captures of Rapture specimens at Syuen's command. To facilitate this, Mihara has the ability to swap her senses with a target of her choice, making it so that if Mihara is injured or hurt, the target will feel the pain instead. This goes both ways, however, as the pain of any injury the target suffers will transfer over to Mihara, and can overwhelm her if she isn't careful. This essentially makes her the "other half" to her fellow Wardress Nikke Yuni, who relishes in causing pain to others. Mihara loves painful stimuli, especially physical ones. Whether she gets hit, she is enjoying. Believing variety is the spice of life, she likes experiencing new sensations, hoping to derive new pleasures from them. She gels well with Yuni who likes to deliver pain and serves as her guardian, closest companion, and voice of reason. After her memory is wiped, Mihara becomes a blank slate. Her masochistic tendencies have all but vanished, but she still cares about Yuni, even when her thoughts start to spiral. Even though the person closest to her would shoot her in the head, after learning about her past and hearing her message, Mihara was willing to forgive Yuni for all she did to her.

Bay is the captain of Rewind, the Ark's cheerleading squad. As the leader of Rewind, Bay is both tough and fair. She is considerate to her teammates, however is known to be stern should any of them make a mistake or for their wellbeing, within reason. She's willing to help her friends whenever they're in trouble. She suffers from scopophobia, or the fear of being looked at. Though this makes performing in front of others much harder, usually leading to her having panic attacks in the worst cases, she is excellent at holding a crowd's attention and staying upbeat so long as her audience is lively enough. Bay's dream is to restore her squad's reputation.

Clay is a new member of the Rewind Squad. She is a huge fan of the team's leader, Bay, and applied to join the squad after the other members quit due to bad press. Though she is a little clumsy and is prone to making minor mistakes, Clay is a hard worker and strives to improve herself. Clay is the upbeat and energetic new recruit for Rewind. She has always wished to join this squad and strives to live up to the lofty expectations set for her. As expected of a cheerleader, she's full of energy and passion and puts it on full display whenever she performs. She is very friendly and personable, allowing her to attract a lot of attention easily. However, she lacks coordination in most physical tasks, leading her to constantly make mistakes and hurting herself. Despite her shortcomings, Clay is a hard worker and trains whenever she has free time to improve little by little, even though she occasionally goes overboard. Because of her fountain of energy, it's hard for her to relax once she gets going.

Elysion Harper, now known as E.H. is the infamous leader of Heavenly Ascension, an armed group of extremists situated in the Outer Rim. She sees Crow as a friend, despite the latter has never viewing her as such. She is Ingrid's half-sister, and once the original heir to Elysion. Despite her organization's infamy, E.H. never wished harm to people. Despite her infamy, E.H. never wishes any ill intent nor harm on anyone as her motto. She is particularly tasked in using her status as the leader of Heavenly Ascension for altruism by improving the quality of life amongst the denizens of the Outer Rim, having colluded once with Doban to ensure their safety during the Rapture's invasion of the Ark and installed lights within the pitch darkness once the Outer Rim was. Her pursuance in doing what's best for the Outer Rim has led her in dedicating in fighting for its denizens' rights. This stems back from her past when she lost her rights to become Elysion's heir, due to her conflicting ideals with her mother's belief in power as she ventured into the slums herself for the first time. She does not take any betrayal lightly, willing to do anything in her power to get rid of anyone who defected against her. In the aftermath of the invasion, E.H. shows some remorse for her actions as Heavenly Ascension's leader, vowing to seek redemption by converting herself into a Nikke. She shows some gratitude to any kindness she receives from other people.

As one of the most easygoing girls you'll meet, she lives life at her own pace. But on the inside, she's quite the schemer, and her laziness is just an act to get others to let down their guard... sometimes. Her hobbies include taking afternoon naps and fishing. She also loves cats and is often seen curling up with them outside on the grass. She's classmates with Special Week.

A supposed next-generation legendary acupuncturist, Sasami claims herself to be the sole disciple of a master of the field. She also claims her skills to be so great that she is capable of curing any affliction with her needles, and even imbuing her patients with new, untold strength. Sasami will appear and offer to treat the player's trainee with her acupuncture. The player is asked to pick from among a selection of chakra for her to activate with her technique, each of which imbues different bonuses, assuming the treatment is successful. If it fails, the trainee suffers a substantial stat loss and is inflicted with a negative status effect. The player is given the option to stop Sasami from giving her treatment, and if picked, Tazuna Hayakawa will escort her out of the academy.

A reporter for the monthly "Twinkle" magazine. She pours her heart and soul into her reporting for the betterment of the racing world. She has just about as much knowledge as trainers do. When she gets too enthusiastic in her reports, she sometimes exaggerates things, but... it just goes to show how sky-high her hopes are.

Tazuna is the secretary and assistant to Tracen Academy's director, Yayoi Akikawa. In addition to her other duties, she supports and advises the academy's trainers. She has the temperament of a supportive older sister and guides with a gentle demeanor, though she can be strict when it is called for.

Behold! Akikawa is the director of Tracen Academy. Behind her exuberant tone and behavior lies a strong will to support all Umamusume. Her generous out-of-pocket support has earned her the respect and gratitude of the students, as well as some stern talking-tos from her secretary Tazuna. Yayoi is the central figure seen in the mobile game's URA Finale scenario, having inherited the position from her mother. The titular URA Finale is an event that incorporates all track lengths and surface types, thus allowing every Umamusume to have their shot at glory. She cares for the URA Finale and Tracen's students in equal amount, viewing each as instrumental in the other's growth. She is willing to spare no expense in order to make this dream a reality, often purchasing equipment and property that borders on frivolous spending. Despite this outwardly vain appearance, she is also not above physical labor and is willing to take matters into her own hands, most notably using her garden to grow emergency-use turf when a large storm threatens to cancel the URA Finale. This philosophy is referenced during gameplay of the URA Finale scenario by having its final set of 3 races be ran on the trainee's preferred track type. For example, racing on more Medium Turf tracks than anything else during a trainee's career will result in Medium Turf races during the Finale. Following the URA Finale, Yayoi leaves for a long-term business trip and is absent for subsequent scenarios, before reappearing in the Project L'Arc scenario in a supporting role.

A stubborn grandma's girl, she aspires to one day become a magical girl. Hoping to match her grandmother, a self-proclaimed witch, in magical prowess, she truly believes that if she keeps on winning races, her magic will also level up in turn. Since she's still learning magic, she often falls back on the good old-fashioned way of solving problems—with force. When she starts crying, it's hard to get her to stop. The absolute a spoiled brat.

An international student from abroad who is emotionally unaffected and quiet, and who places a high priority on efficiency and winning percentage when it comes to racing– she is often feared because of her "workman's" appearance, but she is obsessed with results in order to fulfill her duty to those who have been closely involved with her. She is obsessed with results... In other words, she has a noble spirit. Every Umamusume are scared of her, even she is pretty chill.

Shuichi Saihara is a student in the Ultimate Academy for Gifted Juveniles and a participant of the School Semester. His title is the Ultimate Detective. According to Tsumugi Shirogane, Shuichi Saihara is a fan of a reality show featuring games known as Danganronpa, created by Team Danganronpa. In his audience tape, it was revealed that he was eager to participate in the show as the Ultimate Detective because he adores a lot of characters with the titles in the past seasons. He is willing to do his best to use the talent to commit an unsolvable murder that will amaze the series' viewers, to the point where he can think of his own execution as the Ultimate Detective. Shuichi's parents are a famous actor and a screenwriter, who went overseas for business, which is why Shuichi began to live with his uncle and his wife. While Shuichi claims there isn't any sad story behind it all, his parents are neglectful towards him, and he smiles bitterly whenever he talks about them. To show his gratitude to his uncle, Shuichi helped him by working as his assistant in his detective agency. While he was only helping out and had no desire to be a detective himself, he learned to be a detective this way. Shuichi's first detective case was unofficial but a tough one, as his classmate asked him to find her miniature pet alligator, and he had to do a lot of research, prepare tools and eventually climb around mountains and swim up rivers. As thanks for finding her pet, Shuichi's classmate later gave him chocolate for Valentine's Day, though he assumed it was done just as friends. Shuichi was mostly happy to be useful and memorized her words "thank you". His first official case was an infidelity case, which was nothing compared to the alligator one.